Summary
Type: SPE
Application #: 300840
Catalog#: 730061 - SPE Cartridges, Chromabond, Cyano, 1ml, PP, 100mg, 100/pk
Detection:
Conditions: Column conditioning:
2 column volumes methanol, then 2 column volumes dist. water, finally 2 column
volumes 0.1 M Tris buffer, adjusted to pH 3 with hydrochloric acid
Sample application:
Apply to the column without vacuum:
1) 1 column volume 0.1 M Tris buffer, adjusted to pH 3 with hydrochloric acid
2) sample
3) 1 column volume 0.1 M Tris buffer, adjusted to pH 3 with hydrochloric acid
then slowly force or aspirate liquid through the column
Column washing:
1 column volume 0.1 M Tris buffer, adjusted to pH 3 with hydrochloric acid,
then 1 column volume dist. water
Elution:
2 – 3 ml methanol / 25 % aqueous ammonia solution (99.5 : 0.5, v/v)
Authors: Macherey-Nagel
Source: LC department